Immunological Memory to Zika Virus in a University Community in Colombia, South America.

Zika virus appeared in South America in 2015, generating alarm worldwide as it causes microcephaly and autoimmunity. This study aims to determine the serological footprint of the incoming epidemic in a student community and to characterize the memory functional cell response during post convalescence. In a cross-sectional study, Zika-specific IgG using LIA immunoassay was found in 328 university students (CI=95%), while in the second phase, the functional cellular memory response for IFN-γ and IL-2 was quantified using post-stimulus ELISpot with inactivated virus, starting with individuals seropositive for Zika and control individuals (seropositive only for Dengue and seronegative for Zika-Dengue). Depending on the antigen used, memory humoral response (IgG) against Zika Virus was observed in >60% of the population; seropositivity for NS1 was 21.1% higher than E antigen with high intensity. The analysis of cell functionality in 22 individuals seropositive for Zika virus revealed either IFN-γ+ or IL-2+ cells in 86.3% of cases (Th1 profile), presenting multifunctionality in 50% (11 individuals), 64% of which presented> 6 SFC/104 PBMCs (>600 SFC/106 PBMC), reflecting memory circulating cells. A good agreement (Kappa= 0.754) was observed between the coexistence of both cellular and humoral responses but not in their intensity.

Successful treatment with hysteroscopy for infertility due to isthmocele and hydrometra secondary to cesarean section: A case report.

BACKGROUND: An isthmocele is a scar defect in the uterine wall due to a cesarean section. Its prevalence is unknown, but it has been associated with secondary infertility. Here, we present a case where the patient suffered from an isthmocele that was associated with persistent hydrometra, which developed during in vitro fertilization. CASE SUMMARY: The patient underwent hysteroscopic surgery, which successfully resolved the isthmocele as well as the hydrometra. Afterward, two high-quality, euploid embryos, determined by morphological assessment and pre-implantation genetic diagnostic testing, were transferred. This resulted in uterine pregnancy, as determined by serum ß-human chorionic gonadotropin levels on day 14 (180 mU/mL) and ultrasound-confirmed presence of a gestational sac with a positive embryocardia at week 6. The pregnancy reached 36 wk without any complications, and the product was born in good health. We report a successful isthmocele treatment in a patient with secondary infertility, in which the isthmocele was the cause of persistent hydrometra. CONCLUSION: Hydrometra caused by secondary cesarean is an infertility factor, which can be corrected by hysteroscopy plus ablation of the isthmocele.

Indoles synthesized from amines via copper catalysis.

N-substituted indoles are synthesized from primary amines through a tandem reaction sequence. Initial condensation of the amine with an α-(o-haloaryl)ketone or aldehyde is followed by intramolecular aryl amination catalyzed by CuI. A variety of anilines and alkyl amines, including those with significant steric demands, are converted to indoles in high yields and with varying indole substitution.

Tratamiento quirúrgico de Fracturas mandibulares en pacientes pediátricos: Revisión Bibliográfica / Surgical treatment of mandibular fractures in pediatric patients

Las fracturas faciales en niños muestran características importantes en lo que respecta a su prevalencia, diagnóstico y tratamiento, las cuales las diferencian de las fracturas en adultos. Por estas razones, el trauma infantil debe ser objeto de especial atención ya que presentan particulares características anatómicas, fisiológicas y psicológicas propias de la edad del paciente. Las fracturas mandibulares infantiles que presenten pequeños desplazamientos pueden ser tratadas conservadoramente, mientras que, aquellas que presenten desplazamientos mayores y que interfieran con la función deben ser tratadas de manera semejante a la de los adultos, esto es con reducción cruenta y estabilización, la misma que puede ser obtenida por medio de dispositivos como placas, alambres de acero, tornillos, tornillos y placas de titanio o mediante sistemas de osteosíntesis resorbibles. Estos diferentes sistemas presentan indicaciones, ventajas y desventajas, las cuales deben ser consideradas a fin de elegir la opción de tratamiento más adecuada.

The facial fractures in children show important characteristics about their prevalence, diagnosis and treatment, which differentiate them from fractures in adults. For these reasons, children trauma should be a subject of special attention to due the anatomical, physiological and psychological considerations of the patient´s age. The children jaw fractures that show little displacements can be treat in a conservative way, while those that show greater displacements and interfering with the function should be treated similarly to that in adults, this is with open reduction and stabilization, using some devices such as plaques, steel wires, screws and titanium plaques or through resorbable osteosynthesis systems. These different systems show indications, advantages and disadvantages, which must be considered in order to choose the most suitable treatment.

Cistos odontogênicos em crianças: análise da descompressão cirúrgica em dois casos / The odontogenic cyst in children: analysis of the surgical decompression in 2 cases

Os cistos odontogênicos apresentam-se associados a uma variedade de situações clínicas que devem ser consideradas durante o plano de tratamento. Patologias odontogênicas apresentam baixa incidência em crianças, sendo a relação com elemento dentário permanente um fator decisivo na propedêutica. Cistos dentígeros (CD) associados ao germe permanente e cistos radiculares (CR) são pouco prevalentes. O tratamento de escolha geralmente é o cirúrgico, visando à integridade do elemento permanente. Nestas condições, a marsupialização apresenta-se como uma opção terapêutica. Uma revisão dos aspectos relevantes no tratamento é descrita por meio de dois casos clínicos.

Odontogenic cysts are generally associated with a variety of clinical situations that should be considered during the planning of treatment. There is a low incidence of these cysts in children, the relationship with the permanent tooth being a major factor in the choice of treatment. Dentigerous cysts associated with permanent teeth and radicular ones have a low prevalence. The choice of treatment is usually surgical, with the aim of achieving integrality of the permanent tooth. A review of the important aspects of the treatment is made and two clinical cases are described.

Fibroma Osificante de Mandíbula: Reporte de un caso Clínico

El fibroma osificante es una lesión benigna de crecimiento y tumefacción deformante de evolución lenta que afecta los maxilares, principalmente en la región posterior de la mandíbula. Se manifiesta con mayor predilección en el género femenino, entre la tercera y cuarta décadas de vida. Radiográficamente, la imagen frecuentemente observada, aparece como un área radiolúcida, unilocular, bien definida y delimitada por un halo radiopaco (esclerótico); ocasionalmente puede presentarse como una imagen radiopaca. El tratamiento es quirúrgico y consiste en la enucleación total de la lesión. Este artículo relata un caso de fibroma osificante, discutiendo sus aspectos clínicos a través de la revisión de la literatura.

Ossifying fibroma is a benign lesion, with slow growth but deforming tumefaction involving the jaws, mainly the posterior region of the mandible. It is more frequent in females among the third and fourth decades of life. Radiologically, it usually appears like a radiolucent unilocular well defined area, limited by a radiopaque (sclerotical) edge; occasionally it appears as a radiopaque image. Its treatment is surgical and consists of the total resection of the lesion. This article reports a case of ossifying fibroma, with discussion of its clinical features through a literature review.

Análisis descriptivo de la reparación ósea asociada a implantes dentales endóseos instalados en perros con técnica sumergida en dos períodos de evaluación / Descriptive analyses of osseous repair related to endosseous dental implant installed in dogs with submerged technique in two evaluation period

Los implantes oseointegrados forman parte de la práctica clínica en diferentes disciplinas de la rehabilitación funcional y estética del hombre donde el área de la odontología no es una excepción; de esta forma, los implantes dentales son una respuesta a la gran demanda de paciente con edentulismo parcial o total. Existe una importante y creciente investigación en implantología, siendo imperativo reconocer las características histológicas de la reparación ósea cuando son instalados implantes dentarios. El objetivo de esta investigación es reconocer las características histológicas descriptivas de la reparación ósea asociada a la instalación de implantes dentales en dos periodos de evaluación. El modelo experimental utilizado en este estudio correspondió al de cinco perros sometidos a la exodoncia de premolares mandibulares bilaterales. Después de tres meses fueron instalados implantes bilaterales con dos diseños diferentes y con diferentes protocolos de carga. Los sacrificios de los animales fueron realizados después de tres y seis semanas de instalados los implantes. El estudio histológico descriptivo se desarrolló en el tejido adyacente a implantes instalados con técnica sumergida. Se observaron características de reparación ósea en los dos periodos de evaluación. El periodo de tres semanas presento tejido mineralizado en contacto con el implante y tejido conectivo no mineralizado en aumento desde la región superior hacia la inferior. Las regiones de reparación ósea fueron relacionadas con fenómenos de angiogénesis adyacentes. A las seis semanas se observo una mayor formación ósea, reemplazando las áreas de tejido conectivo no mineralizado por tejido mineralizado. Se observo también tejido óseo maduro con canales de Havers bien constituidos. Concluimos que existe una secuencia biológica de reparación ósea respetada en los dos periodos de evaluación, donde después de seis semanas es posible observar un tejido óseo bien constituido, teóricamente ...

Osseointegrated implants are a part of clinical practice in different areas of the aesthetic and functional human rehabilitation where dentistry is not an exception; being like that, dental implants are a responses to great necessity of patient with partial or total dental lost. Exist an important and crescent research in implantology, being very important to recognize the histological characteristic of the osseous repair when dental implant are installed. The aim of this research was to recognize the histological descriptive characteristic of the osseous repair in two evaluation period. The experimental model in this research was five dogs submitted to dental extraction of mandibular bilateral bicuspid. After three month were installed bilateral implants with two implants design and different load protocols. The animals' sacrifices were realized three and six weeks after implants installation. The histological evaluation was realized in a tissue of implant with submerged techniques. Was observed osseous repair characteristic for two evaluation period. For three weeks period was observed mineralized tissue in contact with implant and non mineralized connective tissue in increase from superior to inferior areas. Reparation areas were related to adjacent angiogenic phenomena. For sex weeks period, was observed a major osseous formation, change connective tissue for mineralized tissue. Was observed too matures osseous tissue with well formed Havers channel. We conclude that exist a biological sequence of osseous repair present for a two evaluation period, where in a sex weeks is observed osseous tissue with good definition, theoretically capable for stress support.

Reconstrucción de piso de orbita con malla de titanio en trauma de tercio medio facial / Orbital Floor reconstruction with titanium mesh for middle third fracture

El trauma maxilofacial es un área desafiante para cirujanos orales y maxilofaciales. En casos de fractura orbitaria, generalmente existirá un defecto óseo en alguna de sus paredes, determinando la necesidad de reconstrucción. La malla de titanio ofrece importantes ventajas para su manipulación e instalación, permitiendo la fácil adaptación en el lugar requerido. Nuestro objetivo es presentar una serie de caso con fracturas de complejo zigomático orbitario (CZO) y con fractura de complejo naso-orbito- tmoidal (NOE), donde la malla da titanio demostró ser efectiva en el manejo de la reconstrucción orbitaria.

Maxillofacial trauma is a challenge area for oral and maxilofacial surgeons. For orbital fracture, generally existing an osseous defect in some of orbital wall, determining the reconstruction necessity. Titanium mesh has importantadvantage for your manipulation and installation with easy adaptation in the selected place. Our objective was present a cases series with zygomatic complex fracture and with naso-orbito-ethmoidal fracture, when titanium mesh showing efficiency for the management of orbital reconstruction.

Bifosfonatos y osteonecrosis de los maxilares: consideraciones sobre su tratamiento / Biphosphonate and jaws osteonecrosis: considerations about of treatment

Los bifosfonatos son potentes inhibidores de los osteoclastos, son drogas usadas principalmente para el tratamiento de mieloma múltiple y en metástasis óseas de tumores sólidos como el cáncer de mama. En el año 2003 fue relatado el primer caso de osteonecrosis de los maxilares (ONM) asociado al uso de bifosfonatos, sin embargo aun no se ha establecido una relación causa efecto de estas drogas sobre la ONM. La incidencia en tiempos anteriores en relación al uso de bifosfonatos era muy baja, siendo que hoy día alcanza 10 por ciento o más en las patologías mencionadas tratadas con estas drogas. Este artículo tiene por objetivo alertar sobre una posible complicación en pacientes que hacen uso de bifosfonatos, a través de la revisión de la literatura y la presentación de un caso clínico.

Bisphosphonates are strong osteoclastic inhibitor activities, being these drugs usually for treatment of multiple myeloma and bone metastases of solid tumors like breast cancer. The osteonecrosis of the maxillary bones was first described in 2003. The relation of these medicines and maxillary bone osteonecrosis still uncertain. The incidence of this bone necrosis was very low before biphosphonates using. Nowadays this complication reach at least 10 percent of patients in these therapy using. The present work mean to alert for possible complication in biphosphonates using patients, thru a literature review and a clinical case presentation.

Reposición maxilar y reconstrucción con injerto de cresta lliaca: parte 1: indicaciones y técnica quirúrgica / Maxillary repositioning and reconstruction with lliac crest graft: part 1: indications and surgical technique

Las técnicas de implantología actuales, no se limitan a evaluar la instalación de implantes y la rehabilitación protésica, sino que deben abocarse a resolver los problemas integrales de nuestros pacientes. De esta forma, devolver armonía facial perdida debe ser un criterio trascendente en la elección de los tratamientos. Con este principio, la estética se establece como un punto de inicio de nuestras terapias, lo cual lleva obligatoriamente a interrelacionarla con la función del sistema estomatognático. Este artículo tiene como objetivo discutir algunos conceptos asociados a la reconstrucción de maxilares atróficos y establecer su relación con fenómenos de deficiencias dentofaciales. Desarrollamos una revisión de aspectos que consideramos relevantes a la hora de escoger determinados tratamientos y presentamos uno de nuestros casos para ejemplificarlo.

Despite solve the implant installation and prosthesis rehabilitation the actual implant are also used to solve other disorders. This way, restore the facial symmetry lost has to be transcendent criteria when choosing a treatment. With this principle, aesthetic becomes a major point in our therapy, correlating it with the stomatognathic system function. The meaning of the present article is discuss some concepts associated with the reconstruction of the atrophic maxillas and established its relation with dentofacial deformities. It was performed a literature review of some points that we consider relevant when choosing a treatment planning and showing one of ours clinical case to exemplify.

Inflammatory fibrous hyperplasia treated with a modified vestibuloplasty: a case report.

AIM: The purpose of this report is to present a case of surgical and prosthetic treatment of a woman with inflammatory fibrous hyperplasia (IFH) and her evaluation during a six month period. BACKGROUND: IFH is a benign pathology, prevalent in female patients, and principally associated with ill-fitting prosthetic devices in need of adjustment. It is common for patients to require surgical removal of the hyperplastic tissue and fabrication of a new prosthesis. CASE REPORT: A 55-year-old female with a history of smoking presented with a chief complaint of missing the scheduled adjustment of her maxillary complete denture and the presence of moveable tissue under the denture. Surgical excision of the hyperplastic tissue followed with fixation of the prosthesis for six months to guide the healing of the soft tissue and to reshape the contours of the maxillary supporting tissues. SUMMARY: Surgical removal of hyperplasic soft tissue is a routine procedure, and the fixation of the prosthesis for the support of tissue during healing improves intraoral conditions for the fabrication of a new prosthesis in the future.

Recuperación Ósea en Procedimientos de Reconstrucción y Colocación de Implantes / Osseous Recovery in Implant Insertion and Pre Implant Reconstructions

La implantología ha evolucionado rápidamente en los últimos 40 años y el empleo de biomateriales en la recuperación de tejidos es cada vez más frecuente. Por esta razón, es necesario conocer el proceso de reparación y la reacción producida entre los los tejidos huéspedes y los biomateriales a nivel histológico, ya que ello contribuirá al éxito, en el largo plazo, de los tratamiento rehabilitadores. Clínicamente, se ha podido observar la efectividad de diferentes compuestos, pero cuando han sido analizados histológicamente, han demostrado algunas variaciones que, muchas veces, las hacen inaceptables para la terapia odontológica. Los objetivos de esta revisión es conocer la forma en que se produce la cicatrización ósea en torno a diferentes biomateriales y como se desarrolla la cicatrización ósea en rehabilitación sobre implantes con diferentes protocolos de carga.

Implantology had experienced a rapid evolution in the last 30 years and the use of biomaterials on tissue recuperation has been more frequent; for this reason it is necessary to know the repaired process and histological reaction between the hosted tissue and the biomaterials, thereby determining the success of the therapy in the long time. Clinically, it has been to possible to see the effects of different biomaterials, but after an histological analyses, has been demonstrated some variations that make them unaccepted for dental treatment. The aim of this review is to report the way of the bone cicatrisation with different biomaterials and the form in which it is developed this cicatrisation on the rehabilitation with implants with different load protocols.